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Effect of ultraviolet irradiation and chlorination on ampicillin-resistant

Yuchen PANG,Jingjing HUANG,Jinying XI,Hongying HU,Yun ZHU

《环境科学与工程前沿（英文）》 2016年 第10卷 第3期   页码 522-530 doi: 10.1007/s11783-015-0779-9

摘要： Antibiotic resistance is a serious public health risk that may spread via potable and reclaimed water. Effective disinfection is important for inactivation of antibiotic-resistant bacteria and disruption of antibiotic resistance genes. Ampicillin is a widely prescribed antibiotic but its effectiveness is increasingly undermined by resistance. In this study, changes in ampicillin resistance for ( ) CGMCC 1.1595 were analyzed after exposure to different doses of ultraviolet (UV) or chlorine, and damage incurred by the plasmid encoding ampicillin resistance gene was assessed. We reported a greater stability in ampicillin-resistant CGMCC 1.1595 after UV irradiation or chlorination when compared with previously published data for other strains. UV irradiation and chlorination led to a shift in the mortality frequency distributions of ampicillin-resistant when subsequently exposed to ampicillin. The ampicillin hemi-inhibitory concentration (IC ) without disinfection was 3800 mg·L , and an increment was observed after UV irradiation or chlorination. The IC of ampicillin-resistant was 1.5-fold higher at a UV dose of 40 mJ·cm , and was 1.4-fold higher when exposed to 2.0 mg·L chlorine. These results indicate that UV irradiation and chlorination can potentially increase the risk of selection for strains with high ampicillin resistance. There was no evident damage to after 1–10 mg Cl ·L chlorination, while a UV dose of 80 mJ·cm yielded a damage ratio for of approximately 1.2-log. Therefore, high UV doses are required for effective disruption of antibiotic resistance genes in bacteria.

关键词： antibiotic resistance     Escherichia coli     ampicillin resistance gene     ultraviolet irradiation     chlorination    

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Assessment of antibiotic resistance genes in dialysis water treatment processes

Xuan Zhu, Chengsong Ye, Yuxin Wang, Lihua Chen, Lin Feng

《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1136-1

摘要：

• Quantitative global ARGs profile in dialysis water was investigated.

• Totally 35 ARGs were found in the dialysis treatment train.

• 29 ARGs (highest) were found in carbon filtration effluent.

• erm and mtrD-02 occurred in the final effluent.

• The effluent was associated with health risks even after RO treatment.

关键词： Dialysis water     Treatment process     Antibiotic resistance gene     High-throughput quantitative PCR     Horizontal gene transfer    

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The antibiotic resistome: gene flow in environments, animals and human beings

null

《医学前沿（英文）》 2017年 第11卷 第2期   页码 161-168 doi: 10.1007/s11684-017-0531-x

摘要：

The antibiotic resistance is natural in bacteria and predates the human use of antibiotics. Numerous antibiotic resistance genes (ARGs) have been discovered to confer resistance to a wide range of antibiotics. The ARGs in natural environments are highly integrated and tightly regulated in specific bacterial metabolic networks. However, the antibiotic selection pressure conferred by the use of antibiotics in both human medicine and agriculture practice leads to a significant increase of antibiotic resistance and a steady accumulation of ARGs in bacteria. In this review, we summarized, with an emphasis on an ecological point of view, the important research progress regarding the collective ARGs (antibiotic resistome) in bacterial communities of natural environments, human and animals, i.e., in the one health settings. We propose that the resistance gene flow in nature is “from the natural environments” and “to the natural environments”; human and animals, as intermediate recipients and disseminators, contribute greatly to such a resistance gene “circulation.”

关键词： antibiotic resistance     resistome     microbiome     gene flow    

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Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline

Mengli Wang, Ruying Li, Qing Zhao

《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1127-2

摘要：

Sludge digestion is critical to control the spread of ARGs from wastewater to soil.

Fate of ARGs in three pretreatment-AD processes was investigated.

UP was more efficient for ARGs removal than AP and THP in pretreatment-AD process.

The total ARGs concentration showed significant correlation with 16S rRNA gene.

The bacteria carrying ARGs could be mainly affiliated with Proteobacteria.

关键词： Tetracycline resistance genes     Sulfonamide resistance genes     16S rRNA gene     Sludge pretreatment     Anaerobic sludge digestion    

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Bacteriophages in water pollution control: Advantages and limitations

《环境科学与工程前沿（英文）》 2021年 第15卷 第5期 doi: 10.1007/s11783-020-1378-y

摘要：

•Phages can be better indicators of enteric viruses than fecal indicator bacteria.

关键词： Phage     Water pollution monitoring     Harmful bacteria biocontrol     Horizontal gene transfer     Bacterial resistance    

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Mitigating microbiological risks of potential pathogens carrying antibiotic resistance genes and virulence

《环境科学与工程前沿（英文）》 2023年 第17卷 第7期 doi: 10.1007/s11783-023-1682-4

摘要：

● Abundance of MAGs carrying ARG-VF pairs unchanged in rivers after WWTP upgrade.

关键词： Wastewater treatment plant upgrade     Antibiotic resistance genes (ARGs)     Virulence factors (VFs)     Gene co-occurrence     Genome-centric analysis    

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Identification of molecular markers linked to rice bacterial blight resistance genes from

Jing WANG,Chen CHENG,Yanru ZHOU,Yong YANG,Qiong MEI,Junmin LI,Ye CHENG,Chengqi YAN,Jianping CHEN

《农业科学与工程前沿（英文）》 2015年 第2卷 第3期   页码 260-265 doi: 10.15302/J-FASE-2015070

摘要： Y73 is a progeny of asymmetric somatic hybridization between cv. Dalixiang and the wild rice species . Inoculation with a range of strains of pv. showed that Y73 had inherited a high level of resistance to rice bacterial blight (BB) from its wild parent. An F population of 7125 individuals was constructed from the cross between Y73 and a BB-susceptible cultivar IR24. After testing 615 SSR and STS markers covering the 12 rice chromosomes, 186 markers were selected that showed polymorphism between Y73 and IR24. Molecular markers linked to the BB resistance genes in Y73 were scanned using the F population and the polymorphic markers. The SSR marker RM128 on chromosome 1, the STS marker R03D159 on chromosome 3 and the STS marker R05D104 on chromosome 5 were found to be linked to the rice BB resistance genes in Y73.

关键词： Oryza meyeriana     bacterial blight resistance gene     genetic population     linkage molecular marker    

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Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells

LI Yahui, DONG Shiyuan, YU Chao, JIANG Yu, LI Huaixing, SUN Shuhan

《医学前沿（英文）》 2007年 第1卷 第3期   页码 269-273 doi: 10.1007/s11684-007-0051-1

摘要： The aim of this article was to investigate the influence and the related mechanism of the gene on glucose uptake and insulin resistance in 3T3-L1 cells. Radioimmunoassay was used to determine glucose uptake in 3T3-L1 cells with different gene expression levels, whether cells were stimulated by insulin or not. RT-PCR and real-time RT-PCR analysis were used to determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different gene expression levels, including insulin receptor substrate-1(IRS-1), phosphatidylinositol 3-kinase (PI-3K), AKT-2, glucose transporter-4 (GLUT-4), p38 mitogen-activated protein kinase (p38MAPK) and glycogen synthase kinase-3b (GSK-3). The glucose uptake decreased with the increase in gene expression in 3T3-L1 cells, which was independent of whether the cells were stimulated by insulin or not. The mRNA expression of two signal proteins PI-3K and AKT-2 decreased and the other two signal proteins, GSK-3 and p38MAPK, increased with overexpression in 3T3-L1 cells. Resistin could induce insulin resistance in adipocytes, which might be related to the changes of some proteins in PI-3K and Ras pathways.

关键词： 3T3-L1     influence     resistance     receptor substrate-1     transport    

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Biofiltration and disinfection codetermine the bacterial antibiotic resistome in drinking water: A review and meta-analysis

Kun Wan, Wenfang Lin, Shuai Zhu, Shenghua Zhang, Xin Yu

《环境科学与工程前沿（英文）》 2020年 第14卷 第1期 doi: 10.1007/s11783-019-1189-1

摘要： Published data was used to analyze the fate of ARGs in water treatment. Biomass removal leads to the reduction in absolute abundance of ARGs. Mechanism that filter biofilm maintain ARB/ARGs was summarized. Potential BAR risks caused by biofiltration and chlorination were proposed. The bacterial antibiotic resistome (BAR) is one of the most serious contemporary medical challenges. The BAR problem in drinking water is receiving growing attention. In this study, we focused on the distribution, changes, and health risks of the BAR throughout the drinking water treatment system. We extracted the antibiotic resistance gene (ARG) data from recent publications and analyzed ARG profiles based on diversity, absolute abundance, and relative abundance. The absolute abundance of ARG was found to decrease with water treatment processes and was positively correlated with the abundance of 16S rRNA (r2 = 0.963, p<0.001), indicating that the reduction of ARG concentration was accompanied by decreasing biomass. Among treatment processes, biofiltration and chlorination were discovered to play important roles in shaping the bacterial antibiotic resistome. Chlorination exhibited positive effects in controlling the diversity of ARG, while biofiltration, especially granular activated carbon filtration, increased the diversity of ARG. Both biofiltration and chlorination altered the structure of the resistome by affecting relative ARG abundance. In addition, we analyzed the mechanism behind the impact of biofiltration and chlorination on the bacterial antibiotic resistome. By intercepting influent ARG-carrying bacteria, biofilters can enrich various ARGs and maintain ARGs in biofilm. Chlorination further selects bacteria co-resistant to chlorine and antibiotics. Finally, we proposed the BAR health risks caused by biofiltration and chlorination in water treatment. To reduce potential BAR risk in drinking water, membrane filtration technology and water boiling are recommended at the point of use.

关键词： Drinking water treatment     Antibiotic resistance gene     Biofiltration     Chlorination    

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ARGs-OAP v3.0——抗生素耐药基因数据库的筛选和分析流程优化 Article

Xiaole Yin, Xiawan Zheng, Liguan Li, An-Ni Zhang, Xiao-Tao Jiang, Tong Zhang

《工程（英文）》 2023年 第27卷 第8期   页码 234-241 doi: 10.1016/j.eng.2022.10.011

摘要：

Antibiotic resistance, which is encoded by antibiotic-resistance genes (ARGs), has proliferated to become a growing threat to public health around the world. With technical advances, especially in the popularization of metagenomic sequencing, scientists have gained the ability to decipher the profiles of ARGs in diverse samples with high accuracy at an accelerated speed. To analyze thousands of ARGs in a highthroughput way, standardized and integrated pipelines are needed. The new version (v3.0) of the widely used ARGs online analysis pipeline (ARGs-OAP) has made significant improvements to both the reference database—the structured ARGs (SARG) database—and the integrated analysis pipeline. SARG has been enhanced with sequence curation to improve annotation reliability, incorporate emerging resistance genotypes, and determine rigorous mechanism classification. The database has been further organized and visualized online in the format of a tree-like structure with a dictionary. It has also been divided into sub-databases for different application scenarios. In addition, the ARGs-OAP has been improved with adjusted quantification methods, simplified tool implementation, and multiple functions with userdefined reference databases. Moreover, the online platform now provides a diverse biostatistical analysis workflow with visualization packages for the efficient interpretation of ARG profiles. The ARGs-OAP v3.0 with an improved database and analysis pipeline will benefit academia, governmental management, and consultation regarding risk assessment of the environmental prevalence of ARGs.

关键词： SARG database ARGs-OAP     Antibiotic-resistance genes     Environmental metagenome     Quantification    

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越南广平和顺化两省猪场分离副猪嗜血杆菌的抗生素耐药表型和耐药基因之间的相关性研究 Article

Chao Nguyen Van, 张丽君, Tam Vu Thi Thanh, Hung Pham Hoang Son, Tuan Tran Ngoc, 黄琦, 周锐

《工程（英文）》 2020年 第6卷 第1期   页码 40-48 doi: 10.1016/j.eng.2019.10.014

摘要：

副猪嗜血杆菌是一种对全球养猪业造成重大经济损失的病原菌。副猪嗜血杆菌防控失败的原因之一是细菌耐药性不断加重。越南拥有亚洲排名第二的生猪产量，但是缺乏针对副猪嗜血杆菌耐药性的相关研究和数据。本研究的目的是调查越南副猪嗜血杆菌的耐药性，并分析耐药性和耐药性基因之间的关系。本研究所使用的副猪嗜血杆菌分离菌株来自我们之前研究中从越南广平省和顺化省猪场分离到的菌株。本研究分别利用肉汤微稀释法和聚合酶链反应（PCR）法检测了这些菌株对25种抗生素的耐药性及相关耐药基因的存在情况。结果显示，这些分离株对甲氧苄啶/磺胺甲恶唑的耐药率达高到94.6%，其次是黏菌素、氯霉素、庆大霉素、青霉素、林可霉素和阿莫西林。这些菌株中相关联耐药基因的检出率分别为blaTEM-1 94.6%、int 76.8%、gyrA 58.9%以及rmtD 50.0%。头孢呋辛、氯霉素和妥布霉素的耐药性表型分别与耐药基因blaROB-1 [优势比（OR） = 26.3, 95% 置信区间（CI）2.7~255.7, p = 0.002]、catl (OR = 25.1, 95% CI 2.4~258.9, p = 0.004)以及strB (OR = 23.5, 95% CI 2.6~212.6, p = 0.001)呈显著正相关。本研究首次揭示了越南中部地区副猪嗜血杆菌的耐药性情况，有助于该疾病的临床控制，能够为降低越南中部的养猪业中该菌的耐药性以及为相关政策制定和临床用药提供指导。

关键词： 副猪嗜血杆菌，抗药性，耐药基因，越南    

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人类遗传病的家系收集疾病基因定位克隆与疾病基因功能的研究

夏家辉

《中国工程科学》 2000年 第2卷 第11期   页码 1-11

摘要：

介绍了中国医学遗传学国家重点实验室在遗传病家系收集、疾病基因定位、疾病基因克隆和疾病基因功能研究方面的研究工作。用细胞遗传学G显带技术于1975年发现了一条与鼻咽癌相关的标记染色体t（1；3）（q44；p11）；1981年将睾丸决定基因（TDF）定位于Yp11.32带；1991年以来收集遗传病家系345种共590个；1996年用显微切割、PCR、微克隆技术克隆了EXT2基因；1998年用基因家族-候选疾病基因克隆方法克隆了遗传性神经性耳聋基因GJB3；1999年用连锁分析和全基因组扫描将一种遗传性弥漫性浅表性光敏性汗孔角化症定位于12q23.2带，并在基因功能研究中发现了一个新的细胞内转运蛋白。

关键词： 遗传病家系     基因定位和克隆     基因家族-候选疾病基因克隆     基因组扫描     基因功能研究    

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Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

《化学科学与工程前沿（英文）》 2017年 第11卷 第4期   页码 663-675 doi: 10.1007/s11705-017-1623-5

摘要： Small interfering RNA (siRNA) therapeutics hold great promise to treat a variety of diseases, as long as they can be delivered safely and effectively into cells. Dendrimers are appealing vectors for siRNA delivery by virtue of their well-defined molecular architecture and multivalent cooperativity. However, the clinical translation of RNA therapeutics mediated by dendrimer delivery is hampered by the lack of dendrimers that are of high quality to meet good manufacturing practice standard. In this context, we have developed small amphiphilic dendrimers that self-assemble into supramolecular structures, which mimic high-generation dendrimers synthesized with covalent construction, yet are easy to produce in large amount and superior quality. Indeed, the concept of supramolecular dendrimers has proved to be very promising, and has opened up a new avenue for dendrimer-mediated siRNA delivery. A series of self-assembling supramolecular dendrimers have consequently been established, some of them out-performing the currently available nonviral vectors in delivering siRNA to various cell types and , including human primary cells and stem cells. This short review presents a brief introduction to RNAi therapeutics, the obstacles to their delivery and the advantages of dendrimer delivery vectors as well as our bio-inspired structurally flexible dendrimers for siRNA delivery. We then highlight our efforts in creating self-assembling amphiphilic dendrimers to construct supramolecular dendrimer nanosystems for effective siRNA delivery as well as the related structural alterations to enhance delivery efficiency. The advent of self-assembling supramolecular dendrimer nanovectors holds great promise and heralds a new era of dendrimer-mediated delivery of RNA therapeutics in biomedical applications.

关键词： gene therapy     RNAi therapeutics     dendrimer     nanovectors     gene silencing    

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Reflections on the system of evaluation of gene-edited livestock

Ziyao FAN, Tianwen WU, Kui WU, Yulian MU, Kui LI

《农业科学与工程前沿（英文）》 2020年 第7卷 第2期   页码 211-217 doi: 10.15302/J-FASE-2019303

摘要：

The rapid development of biotechnology has provided a greater understanding of the biological functions of major candidate genes that have important functions regarding economic traits, and new materials for livestock breeding have been obtained through gene editing (GE) and embryo manipulation with the purpose of improving quality and output and reducing the costs and risk of disease. Public concerns, particularly over safety risks and production performance, must be addressed. Evaluation is the most important component of the regulation of gene-edited livestock and is a crucial guarantee of public safety before the marketing of gene-edited animal products. Here, the system of evaluation of gene-edited livestock is discussed in terms of public safety and production performance. The search for safe and ethical applications in the GE of livestock, a case-by-case evaluation strategy, and classification and simplification are used in order to promote a more efficient, objective, comprehensive and operable evaluation system.

关键词： evaluation     gene editing     livestock     performance     safety    

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Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation

《医学前沿（英文）》 2022年 第16卷 第4期   页码 627-636 doi: 10.1007/s11684-020-0815-4

摘要： Runt-related transcription factor 1 (RUNX1) is an essential regulator of normal hematopoiesis. Its dysfunction, caused by either fusions or mutations, is frequently reported in acute myeloid leukemia (AML). However, RUNX1 mutations have been largely under-explored compared with RUNX1 fusions mainly due to their elusive genetic characteristics. Here, based on 1741 patients with AML, we report a unique expression pattern associated with RUNX1 mutations in AML. This expression pattern was coordinated by target repression and promoter hypermethylation. We first reanalyzed a joint AML cohort that consisted of three public cohorts and found that RUNX1 mutations were mainly distributed in the Runt domain and almost mutually exclusive with NPM1 mutations. Then, based on RNA-seq data from The Cancer Genome Atlas AML cohort, we developed a 300-gene signature that significantly distinguished the patients with RUNX1 mutations from those with other AML subtypes. Furthermore, we explored the mechanisms underlying this signature from the transcriptional and epigenetic levels. Using chromatin immunoprecipitation sequencing data, we found that RUNX1 target genes tended to be repressed in patients with RUNX1 mutations. Through the integration of DNA methylation array data, we illustrated that hypermethylation on the promoter regions of RUNX1-regulated genes also contributed to dysregulation in RUNX1-mutated AML. This study revealed the distinct gene expression pattern of RUNX1 mutations and the underlying mechanisms in AML development.

关键词： RUNX1     gene mutation     acute myeloid leukemia     transcriptional repression     DNA methylation    

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标题 作者 时间 类型 操作

Effect of ultraviolet irradiation and chlorination on ampicillin-resistant

Yuchen PANG,Jingjing HUANG,Jinying XI,Hongying HU,Yun ZHU

期刊论文

Assessment of antibiotic resistance genes in dialysis water treatment processes

Xuan Zhu, Chengsong Ye, Yuxin Wang, Lihua Chen, Lin Feng

期刊论文

The antibiotic resistome: gene flow in environments, animals and human beings

null

期刊论文

Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline

Mengli Wang, Ruying Li, Qing Zhao

期刊论文

Bacteriophages in water pollution control: Advantages and limitations

期刊论文

Mitigating microbiological risks of potential pathogens carrying antibiotic resistance genes and virulence

期刊论文

Identification of molecular markers linked to rice bacterial blight resistance genes from

Jing WANG,Chen CHENG,Yanru ZHOU,Yong YANG,Qiong MEI,Junmin LI,Ye CHENG,Chengqi YAN,Jianping CHEN

期刊论文

Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells

LI Yahui, DONG Shiyuan, YU Chao, JIANG Yu, LI Huaixing, SUN Shuhan

期刊论文

Biofiltration and disinfection codetermine the bacterial antibiotic resistome in drinking water: A review and meta-analysis

Kun Wan, Wenfang Lin, Shuai Zhu, Shenghua Zhang, Xin Yu

期刊论文

ARGs-OAP v3.0——抗生素耐药基因数据库的筛选和分析流程优化

Xiaole Yin, Xiawan Zheng, Liguan Li, An-Ni Zhang, Xiao-Tao Jiang, Tong Zhang

期刊论文

越南广平和顺化两省猪场分离副猪嗜血杆菌的抗生素耐药表型和耐药基因之间的相关性研究

Chao Nguyen Van, 张丽君, Tam Vu Thi Thanh, Hung Pham Hoang Son, Tuan Tran Ngoc, 黄琦, 周锐

期刊论文

人类遗传病的家系收集疾病基因定位克隆与疾病基因功能的研究

夏家辉

期刊论文

Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

期刊论文

Reflections on the system of evaluation of gene-edited livestock

Ziyao FAN, Tianwen WU, Kui WU, Yulian MU, Kui LI

期刊论文

Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation

期刊论文